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mab860 55d  (R&D Systems)


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    R&D Systems mab860 55d
    Fig. 6. AXL interacts with GST-VP1u. (A) Immunofluorescent assay for colocalization of AXL with B19V virions. CD36+ EPCs cells were infected with B19V at an MOI of ~5000 vgc per cell for 1.5 hours at 4°C. The cells were then washed with ice-cold PBS, then cytospun onto slides, fixed, permeabilized, and stained with an anti-B19V capsid antibody (clone <t>MAB860-55D).</t> Confocal images were taken under a confocal microscope. Nuclei were stained with DAPI (blue). Scale bar, 10 μm. (B to D) Kinetics of the in vitro interaction between AXL and VP1u using Ni-NTA biosensors. The binding kinetics depicts the associations and dissociations of 4 μM rAXLEC with GST-VP1u at the indicated concentrations (B). Binding kinetics of 4 μM AXLHis with 4 and 8 μM GST was used as a control (C). The binding parameters of AXL and GST-VP1u are shown (D). Equilibrium dissociation constant KD value represents the ratio of dissociation [Kd (1/s)] and association [Ka (1/Ms)] computed from the real-time binding curves of rAXLEC to GST-VP1u. The values are shown with means ± SDs based on at least three repeated experiments.
    Mab860 55d, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mab860 55d/product/R&D Systems
    Average 94 stars, based on 37 article reviews
    mab860 55d - by Bioz Stars, 2026-06
    94/100 stars

    Images

    1) Product Images from "Identification of AXL as a co-receptor for human parvovirus B19 infection of human erythroid progenitors."

    Article Title: Identification of AXL as a co-receptor for human parvovirus B19 infection of human erythroid progenitors.

    Journal: Science advances

    doi: 10.1126/sciadv.ade0869

    Fig. 6. AXL interacts with GST-VP1u. (A) Immunofluorescent assay for colocalization of AXL with B19V virions. CD36+ EPCs cells were infected with B19V at an MOI of ~5000 vgc per cell for 1.5 hours at 4°C. The cells were then washed with ice-cold PBS, then cytospun onto slides, fixed, permeabilized, and stained with an anti-B19V capsid antibody (clone MAB860-55D). Confocal images were taken under a confocal microscope. Nuclei were stained with DAPI (blue). Scale bar, 10 μm. (B to D) Kinetics of the in vitro interaction between AXL and VP1u using Ni-NTA biosensors. The binding kinetics depicts the associations and dissociations of 4 μM rAXLEC with GST-VP1u at the indicated concentrations (B). Binding kinetics of 4 μM AXLHis with 4 and 8 μM GST was used as a control (C). The binding parameters of AXL and GST-VP1u are shown (D). Equilibrium dissociation constant KD value represents the ratio of dissociation [Kd (1/s)] and association [Ka (1/Ms)] computed from the real-time binding curves of rAXLEC to GST-VP1u. The values are shown with means ± SDs based on at least three repeated experiments.
    Figure Legend Snippet: Fig. 6. AXL interacts with GST-VP1u. (A) Immunofluorescent assay for colocalization of AXL with B19V virions. CD36+ EPCs cells were infected with B19V at an MOI of ~5000 vgc per cell for 1.5 hours at 4°C. The cells were then washed with ice-cold PBS, then cytospun onto slides, fixed, permeabilized, and stained with an anti-B19V capsid antibody (clone MAB860-55D). Confocal images were taken under a confocal microscope. Nuclei were stained with DAPI (blue). Scale bar, 10 μm. (B to D) Kinetics of the in vitro interaction between AXL and VP1u using Ni-NTA biosensors. The binding kinetics depicts the associations and dissociations of 4 μM rAXLEC with GST-VP1u at the indicated concentrations (B). Binding kinetics of 4 μM AXLHis with 4 and 8 μM GST was used as a control (C). The binding parameters of AXL and GST-VP1u are shown (D). Equilibrium dissociation constant KD value represents the ratio of dissociation [Kd (1/s)] and association [Ka (1/Ms)] computed from the real-time binding curves of rAXLEC to GST-VP1u. The values are shown with means ± SDs based on at least three repeated experiments.

    Techniques Used: Infection, Staining, Microscopy, In Vitro, Binding Assay, Control



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    mab860 55d  (R&D Systems)
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    R&D Systems mab860 55d
    Fig. 6. AXL interacts with GST-VP1u. (A) Immunofluorescent assay for colocalization of AXL with B19V virions. CD36+ EPCs cells were infected with B19V at an MOI of ~5000 vgc per cell for 1.5 hours at 4°C. The cells were then washed with ice-cold PBS, then cytospun onto slides, fixed, permeabilized, and stained with an anti-B19V capsid antibody (clone <t>MAB860-55D).</t> Confocal images were taken under a confocal microscope. Nuclei were stained with DAPI (blue). Scale bar, 10 μm. (B to D) Kinetics of the in vitro interaction between AXL and VP1u using Ni-NTA biosensors. The binding kinetics depicts the associations and dissociations of 4 μM rAXLEC with GST-VP1u at the indicated concentrations (B). Binding kinetics of 4 μM AXLHis with 4 and 8 μM GST was used as a control (C). The binding parameters of AXL and GST-VP1u are shown (D). Equilibrium dissociation constant KD value represents the ratio of dissociation [Kd (1/s)] and association [Ka (1/Ms)] computed from the real-time binding curves of rAXLEC to GST-VP1u. The values are shown with means ± SDs based on at least three repeated experiments.
    Mab860 55d, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mab860 55d/product/R&D Systems
    Average 94 stars, based on 1 article reviews
    mab860 55d - by Bioz Stars, 2026-06
    94/100 stars
    		  Buy from Supplier

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    Fig. 6. AXL interacts with GST-VP1u. (A) Immunofluorescent assay for colocalization of AXL with B19V virions. CD36+ EPCs cells were infected with B19V at an MOI of ~5000 vgc per cell for 1.5 hours at 4°C. The cells were then washed with ice-cold PBS, then cytospun onto slides, fixed, permeabilized, and stained with an anti-B19V capsid antibody (clone MAB860-55D). Confocal images were taken under a confocal microscope. Nuclei were stained with DAPI (blue). Scale bar, 10 μm. (B to D) Kinetics of the in vitro interaction between AXL and VP1u using Ni-NTA biosensors. The binding kinetics depicts the associations and dissociations of 4 μM rAXLEC with GST-VP1u at the indicated concentrations (B). Binding kinetics of 4 μM AXLHis with 4 and 8 μM GST was used as a control (C). The binding parameters of AXL and GST-VP1u are shown (D). Equilibrium dissociation constant KD value represents the ratio of dissociation [Kd (1/s)] and association [Ka (1/Ms)] computed from the real-time binding curves of rAXLEC to GST-VP1u. The values are shown with means ± SDs based on at least three repeated experiments.

    Journal: Science advances

    Article Title: Identification of AXL as a co-receptor for human parvovirus B19 infection of human erythroid progenitors.

    doi: 10.1126/sciadv.ade0869

    Figure Lengend Snippet: Fig. 6. AXL interacts with GST-VP1u. (A) Immunofluorescent assay for colocalization of AXL with B19V virions. CD36+ EPCs cells were infected with B19V at an MOI of ~5000 vgc per cell for 1.5 hours at 4°C. The cells were then washed with ice-cold PBS, then cytospun onto slides, fixed, permeabilized, and stained with an anti-B19V capsid antibody (clone MAB860-55D). Confocal images were taken under a confocal microscope. Nuclei were stained with DAPI (blue). Scale bar, 10 μm. (B to D) Kinetics of the in vitro interaction between AXL and VP1u using Ni-NTA biosensors. The binding kinetics depicts the associations and dissociations of 4 μM rAXLEC with GST-VP1u at the indicated concentrations (B). Binding kinetics of 4 μM AXLHis with 4 and 8 μM GST was used as a control (C). The binding parameters of AXL and GST-VP1u are shown (D). Equilibrium dissociation constant KD value represents the ratio of dissociation [Kd (1/s)] and association [Ka (1/Ms)] computed from the real-time binding curves of rAXLEC to GST-VP1u. The values are shown with means ± SDs based on at least three repeated experiments.

    Article Snippet: The fixed cells were then incubated with an anti-B19V capsid antibody [#MAB8292/clone 521-5D (40) or #MAb860-55D (25, 62)] to detect intact B19V virions or costained with anti-AXL antibody (#AF154, R&D Systems).

    Techniques: Infection, Staining, Microscopy, In Vitro, Binding Assay, Control